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MacVector 10 Screencasts
The following videos show aspects of some of the new features of MacVector 10. As we get closer to the release we'll be adding more videos. Please check back regularly as for new screencasts. (Note: if you experience jerky playback, click pause and wait until the video downloads fully before playback).
New Sequence Window overview
To reduce screen clutter and simplify the interface, many of the common windows in MacVector 10.0 have been combined into a single window with tabbed views. For most windows, a “Replica” toolbar button lets you open a second window for those times when you need to see more than one tab at a time.
Primer Design (Primer3) Overview
We've added a new Primer design module. There is a new primer design function in MacVector 10.0. This is based on the popular Primer3 algorithm and is accessed through the Analyze->Primers sub menu.
The default Primer3 implementation lets you choose a region to amplify and will automatically find the best 5 pairs of primers. You can also find primers based on product size or by defining flanking regions.
You can also specify your own primer(s) and have Primer3 evaluate them for suitability.
The results are displayed in tabular and graphical format. The views are interactive and linked to the parent sequence so that you can select primers and/or products and copy/paste to other windows or use to create new features on the sequence.
As well as having increased functionality such as being able to design internal oligos for such analyses as realtime PCR, it's also a lot easier to use. The following screencast shows a basic overview of how designing some primers can be as easy as 3 mouse clicks. We'll be adding some more in depth looks at Primer Design (Primer3) soon.
Automatic Restriction Enzyme Site Searching
We've added a new Automatic Restriction Enzyme Searching to the main Sequence map window. You can now have sequences be automatically scanned for restriction enzymes and have these displayed in the default map view. This is configured from the revised Graphic Options dialog, now accessible from a new Preferences button on the Map tab toolbar. By default, this is set to use selected enzymes in the “Common Enzymes” file.
This dramatically simplifies the use of MacVector for “click cloning”. You can open a source destination sequence, click on the appropriate restriction sites, choose Edit->Copy to copy the fragment, and paste directly into a matching site in a suitable target sequence.
You can easily design a subcloning experiment with MacVector's Click Cloning function. You can find the best way to insert your sequence into a vector, or use MacVector to document your new construct.
This has been improved with MacVector 10 as you can now use the automatic restriction enzyme to make click cloning very simple. The following screencast shows one such quick procedure, as well as showing an overview of the click cloning function.