Tutorials

Getting Started

Use this document if you are a brand new MacVector user and want to know how to get started with basic MacVector functions like opening files, retrieving sequences from online services and performing basic sequence analyses and constructions.

Tutorials

When MacVector is installed on a computer, a collection of tutorials is placed into the /Applications/MacVector/Documentation/ folder. You can also download the latest versions of these.

Basic Techniques

Primer Design

Basic Contig Assembly

Align To Reference – Sequence Confirmation

Sequence Annotation

Automatic Sequence Annotation

Creating Vector Maps

Assembling Plasmids from NGS data

Cloning

Clone Construction

Gateway and TOPO Cloning

Gibson Assembly and Ligase Independent Cloning

Advanced Techniques

Virtual Gene Cloning From RNA-Seq Data Tutorial and you’ll also need to download the sample data set.

Next Generation Sequencing Reference Alignments

RNASeq Expression Analysis

RNASeq Human Transcriptome Analysis Tutorial and you’ll also need to download the sample data set.

Transposon Insertion Analysis Tutorial and you’ll also need to download the sample data set.

Workshop Manual

MacVector staff frequently visit customer sites to present workshops. These typically consist of working through some of the most popular tutorials along with a general overview of functionality as documented in the Getting Started Workshop Manual

MacVector Workshop manual

Technical Notes

Rather than step-by-step guides to MacVector’s functionality, like the tutorials, Technical Notes are a more formal higher level of documentation that illustrates how to use MacVector to perform rigorous scientific analysis of sequences.

Datamining to identify Pangolin SARS-CoV-2 Data mining with MacVector confirms pangolins as potential intermediary hosts during thre evolution of SARS-CoV-2, the coronavirus infectious agent of COVID-19.