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QUICK TIP: Aligning primers against a template 
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Joined: Thu Dec 07, 2006 6:20 pm
Posts: 173
Location: Cambridge, UK
Post QUICK TIP: Aligning primers against a template
If you want to quickly align a large set of primers against a template sequence, then as long as each primer is in a separate MacVector file, you can use Sequence Confirmation.

Open the template file and got to ANALYZE > SEQUENCE CONFIRMATION to open up a new Assembly.
Import your primer sequences.
Go to ASSEMBLE and change these two parameters:


Code:
MATCH to 5
SENSITIVITY to 10


The resulting alignment will show your primers aligned against the template.


Wed Sep 26, 2007 4:46 pm
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