Home MacVector Assembler Downloads Try HowToBuy Support Contact Forums

Creating a Sequencing Project

The first step in any assembly is to create a project and import the sequences/trace files that are to be assembled. MacVector Assembler lets you directly import files in the following formats;

Chromatogram Files

• ABI (from 373, 377, 3700 and 3730 models)

• SCF version 2

• SCF version 3

• ALF

Sequence Files

• MacVector

• FastA

• FastQ

Sequence files in other formats should first be converted into a compatible format using MacVector.

The imported sequences are maintained in a project window where you can sort and rename etc.

PastedGraphic

A populated sequence assembly project

MacVectorInc202px

Copyright © 2012 MacVector, Inc. All rights reserved. Terms of Use.

MacVector, Inc • PMB 150 • 1939 High House Road • Cary • North Carolina 27519 • USA

phone: +1-919-303-7450 • toll free: +1-866-338-0222 • fax: +1-919-303-7449

Overview

Creating a Sequencing Project

Base Calling Using phred

Vector Trimming with cross_match

Assembling Sequences using phrap

Editing and Analysis of Contigs

NGS Reference Assembly using Bowtie

Comparing Assembler and AssemblyLIgn